rat monoclonal tonbo biosciences  (Cytek Biosciences)

Journal: eLife

Article Title: Germinal center B cells that acquire nuclear proteins are specifically suppressed by follicular regulatory T cells

doi: 10.7554/eLife.83908

Figure Lengend Snippet: ( A–G ) Single-cell 10× Genomics (Cell Ranger) analysis of gene expression and TCR repertoire of the CD4 + CD8 - 220 - CXCR5 hi PD1 hi cells (Tfr and Tfh) sorted from the draining inguinal lymph nodes (dLNs) of mice immunized with SA-DEL and boosted with SA (two mice) or SA-NucPr (two mice) as in . ( A ) Graph-based clustering of follicular T cells from SA and SA-NucPr-boosted mice visualized in 2D using uniform manifold approximation and projection for dimension reduction (UMAP) algorithm. ( B, C ) Single-cell expression of Foxp3 (in B ) and Il10 (in C ) in follicular T cells for combined SA and SA-NucPr data shown in UMAP. ( D ) Expression of selected genes associated with Treg-mediated regulation in the follicular T cell clusters 1–12. Clusters 9–12 are enriched for follicular T cells with Foxp3 expression and will be called Tfr-like cell clusters. Clusters 1–8 will be called Tfh-like cell clusters. ( E ) Overlap of TCR clones (with >1 cell per clone) within Tfr-like clusters 9–12 with other follicular T cell clusters (color-coded as in 2A). Note that the largest fraction of TCR clones in cluster 9 overlap with cluster 10 and vice versa. ( F ) Fraction of TCR clones (with >1 cell per clone) within Tfr-like clusters 9–12 that overlap or not with Tfh-like clusters 1–8 after SA versus SA-NucPr boosting. Note that there is no increase in the Tfh-like TCR clones within Tfr-like clones after SA-NucPr boosting. ( G ) Relative abundance of follicular T cells in 1–12 clusters in the SA versus SA-NucPr-boosted mice. Note about twofold increase in the Tfr-like cluster 10 in the SA-NucPr-boosted mice. ( H–K ) Flow cytometry analysis of CD25, CTLA4, and granzyme B expression in CD4 + CD8 - B220 - CXCR5 high PD1 high cells that are FOXP3 - (Tfh), FOXP3 + ICOS high (Tfr ICOS high ) and FOXP3 ICOS int/low (Tfr ICOS int/lo ) in the dLNs of SA-NucPr-boosted mice. ( H ) The gating strategy of CD4 + CD8 - B220 - CXCR5 high PD1 high cells. ( I–K ) Representative flow histograms (left panels) and percent of positive cells (right panel) for CD25 (in I ) or MFIs (right panels) for CTLA4 (in J ), granzyme B (in K ). n=2 independent experiments. Each point represents one mouse. One-way ANOVA with Bonferroni’s multiple comparisons test.

Article Snippet: Antibody , Anti-mouse CD8a Biotin clone 53–6.7 (Rat monoclonal) , BD Bioscience , Cat#553029; PRID:AB 394567 , FC (1:100).

Techniques: Expressing, Clone Assay, Flow Cytometry

Journal: eLife

Article Title: Germinal center B cells that acquire nuclear proteins are specifically suppressed by follicular regulatory T cells

doi: 10.7554/eLife.83908

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-mouse CD8a Biotin clone 53–6.7 (Rat monoclonal) , BD Bioscience , Cat#553029; PRID:AB 394567 , FC (1:100).

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay, Staining, Blocking Assay, Recombinant, Purification, Software

Journal: Journal of visualized experiments : JoVE

Article Title: Analysis of Hematopoietic Stem Progenitor Cell Metabolism

doi: 10.3791/60234

Figure Lengend Snippet: Materials

Article Snippet: Biotin Rat Anti-Mouse CD8a Clone 53-6.7 , BD Biosciences , 553029 , Used for Lin- separation.

Techniques: Filtration, Cell Isolation, Cell Culture

Journal: Cancer cell

Article Title: Eradication of Tumors through Simultaneous Ablation of CD276/B7-H3 Positive Tumor Cells and Tumor Vasculature

doi: 10.1016/j.ccell.2017.03.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat Anti-Mouse CD8a Biotin (clone: 53–6.7) , eBioscience , Cat# 13-0081-82.

Techniques: Control, Purification, Plasmid Preparation, Virus, Recombinant, Electron Microscopy, Transfection, Blocking Assay, In Situ, Labeling, Western Blot, CRISPR, Expressing, Software